Preliminary data on an immunoenzymatic assay of free thyroxin.
نویسندگان
چکیده
منابع مشابه
Evaluation of a new free-thyroxin assay.
The Amerlex Free Thyroxin (T4) Radioimmunoassay Kit (Amersham International Ltd.) is a new direct equilibrium radioimmunoassay for free T4 based on an antiserum with very high affinity for T4, and a unique 125I-labeled T4 analog as tracer. It is a very simple single-tube radioimmunoassay, making use of Amerlex particles to separate antibody-bound from free species. Interassay precision (CV) is ...
متن کاملEffect of albumin on results of an "improved" analog-type assay for free thyroxin.
The paper by Sykes et al. (1) on the effect of phospholipase C on high-molecular-mass alkaline phosphatase in serum contains nomenclatural errors. In the Abstract, an Enzyme Comnussion number (EC 3.1.4.3) is given for the phospholipase C of Bacillus cereus; this is not the Enzyme Commission number of the phospholipase C responsible for the effect reported (i.e., the release of liver alkaline ph...
متن کاملComprehensive study of a thyroxin-analog-based assay for free thyroxin ("Amerlex FT4").
The basic theory of thyroxin-analog-based radioimmunoassays for free thyroxin has been extended to evaluate definitively the effects arising from residual binding of the tracer analog to serum proteins. Using experimentally determined binding constants and computer simulation techniques, we studied the effects of thyroxin-analog binding to serum proteins on results of Amerlex FT4 radioimmunoass...
متن کاملEnzyme-linked immunosorbent assay for free thyroxin in human serum.
We describe a simple enzyme-linked immunosorbent assay (ELISA) in which microtiter plates are used for determining the free thyroxin concentration (FT4) in serum. Only commercially available chemicals and reagents are needed, including the antiserum. The working range is 1 to 60 ng/L. Turnaround time is about 4 h. Within-run coefficients of variation (CV) for FT4 concentrations of 9.0, 19.0, an...
متن کاملOne-step, labeled-antibody assay for measuring free thyroxin. I. Assay development and validation.
We describe a one-step, labeled-antibody radioassay for measuring free thyroxin (FT4) in serum or plasma, based on a novel principle. FT4 in the sample competes with a gross molar excess (over antibody) of a cross-reactant (L-triiodothyronine, T3), chemically coupled to magnetizable polymer particles, for binding to avid 125l-labeled monoclonal anti-thyroxin antibodies. As in conventional immun...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1986
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/32.4.697a